Later in the evening Curtis Cup 18-year-old Sally Watson made it two Scots in the third round with a three-under-par performance.
MacDonald, a 19-year-old Stirling University student from Nairn beat the Spanish ace, Carlota Ciganda by one hole in a match of rare quality.
In the 1st round two other Scots, Curtis Cup player Pamela Pretswell and Robert Gordon University student Jane Turner were beaten.
Pamela shot four birdies but still lost narrowly on the last green to Spanish ace Carlota Ciganda who won the title 3 years ago and lost in the final last year.
Pamela was two up on Ciganda after eight holes but the Spaniard hit a purple patch to turn that around and lead by two holes on the 13th tee.
Ciganda finally knocked out the Scot by holing a 30ft putt at the last for a one-hole victory after a great match. Ciganda had a final tally of one eagle and three birdies. Both players were under par at the finish of quality tie.
"I was beaten on the last but I enjoyed it tremendously playing against such a very good player," said sporting Pamela.
Thanks go to Colin Farquharson for the report
Go to the Gillian Kirkwood Website for the 2nd round Results and reports
Go to the Gillian Kirkwood Website for the 2nd round Results and reports